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Volume 36 Issue 2
Apr 2025
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Article Contents
Journal of Earth Science  > 2025  >  36(2): 395-407.
Hongfei Chi, Xiufeng Yin, Xuefeng Zhang, Jingyi Zhu, Lin Zhao, Yongcui Deng, Pengfei Liu, Yongqin Liu. Influence of Interspecies Interactions on Bacterial Community Assembly in the Active and Permafrost Layers on the Qinghai-Tibet Plateau. Journal of Earth Science, 2025, 36(2): 395-407. doi: 10.1007/s12583-024-0046-8
Citation: Hongfei Chi, Xiufeng Yin, Xuefeng Zhang, Jingyi Zhu, Lin Zhao, Yongcui Deng, Pengfei Liu, Yongqin Liu. Influence of Interspecies Interactions on Bacterial Community Assembly in the Active and Permafrost Layers on the Qinghai-Tibet Plateau. Journal of Earth Science, 2025, 36(2): 395-407. doi: 10.1007/s12583-024-0046-8
shu

Influence of Interspecies Interactions on Bacterial Community Assembly in the Active and Permafrost Layers on the Qinghai-Tibet Plateau

doi: 10.1007/s12583-024-0046-8
  • 1.

    College of Ecology, Lanzhou University, Lanzhou 730000, China

  • 2.

    Center for the Pan-Third Pole Environment, Lanzhou University, Lanzhou 730000, China

  • 3.

    State Key Laboratory of Cryospheric Science, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Science, Lanzhou 730000, China

  • 4.

    State Key Laboratory of Tibetan Plateau Earth System, Resources and Environment (TPESRE), Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China

  • 5.

    College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China

  • 6.

    School of Geographical Sciences, Nanjing University of Information Science and Technology, Nanjing 210044, China

  • 7.

    School of Geography, Nanjing Normal University, Nanjing 210023, China

  • 8.

    Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Nanjing 210023, China

More Information
  • Corresponding author: Pengfei Liu, liupf@lzu.edu.cn; Yongqin Liu, yql@lzu.edu.cn
  • Received Date: 01 Jul 2024
  • Accepted Date: 28 Jul 2024
  • Issue Publish Date: 30 Apr 2025
    Abstract

  • Bacterial communities play a crucial role in permafrost biogeochemical cycling and ecosystem function maintenance. Bacterial interaction is one of the main factors in shaping soil bacterial communities. However, how would bacterial interaction influence the bacterial communities in permafrost of the Qinghai-Tibet Plateau (QTP) remains largely unknown. Here we collected paired soil samples from both the active and permafrost layers of two typical QTP permafrost regions in October 2020 for Tuotuohe River (TTH) and May 2022 for Aerjin (ARJ), and investigated the bacterial communities and the role of interactions in structuring the bacterial community and its assembly process through amplicon sequencing of the 16S rRNA gene. Our study revealed distinct bacterial communities, with significant differences in the relative abundances of Proteobacteria (P < 0.05), Acidobacteriota (P < 0.001), Bacteroidota (P < 0.05), and Planctomycetota (P < 0.001) between the active layer and the permafrost layer. More importantly, we found that interspecies interactions, including both positive and negative associations, were strongly correlated with bacterial alpha-diversity and played a significant role in community variation and assembly process. Our findings also showed that the community assembly in both the active and permafrost layers was primarily driven by homogeneous selection of deterministic processes, with interspecies interactions accounting for more than 58% and 63% of all assembly mechanisms, respectively. This is the first study to quantify the contribution of bacterial interactions in shaping the bacterial community and its assembly process in permafrost of QTP, highlighting the importance of considering interspecies interactions in future modeling efforts. Our work also emphasizes the necessity of including interspecies interactions in microbial process projections to reduce uncertainty.

     

    • Electronic Supplementary Materials: Supplementary materials (Supplementary methods, results and discussion; Tables S1–S2; Figures S1–S8) are available in the online version of this article at https://doi.org/10.1007/s12583-024-0046-8.
    Conflict of Interest
    The authors declare that they have no conflict of interest.
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