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Volume 26 Issue 2
Apr 2015
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Article Contents
Yongqiang Yuan, Jianming Zhu, Congqiang Liu, Shen Yu, Lei Lei. Biomineralization of Se Nanoshpere by Bacillus Licheniformis. Journal of Earth Science, 2015, 26(2): 246-250. doi: 10.1007/s12583-015-0536-9
Citation: Yongqiang Yuan, Jianming Zhu, Congqiang Liu, Shen Yu, Lei Lei. Biomineralization of Se Nanoshpere by Bacillus Licheniformis. Journal of Earth Science, 2015, 26(2): 246-250. doi: 10.1007/s12583-015-0536-9

Biomineralization of Se Nanoshpere by Bacillus Licheniformis

doi: 10.1007/s12583-015-0536-9
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  • Corresponding author: Yongqiang Yuan,
  • Received Date: 18 Mar 2014
  • Accepted Date: 15 Feb 2015
  • Publish Date: 01 Apr 2015
  • Biological dissimilatory reduction of selenite (SeO3 2−) to elemental selenium (Se0) is common,but the mineral formation and the biogenic process remain uncertain. In this study,we examined the Se0 formation during the selenite bioreduction by Bacillus licheniformis SeRB-1 through transmission electron microscope (TEM),energy-dispersive spectrometry (EDS) and X-ray absorption fine structure (XAFS) techniques. Results showed that the reduction process occurred mostly during the exponential phase and early stationary phase,whilst the elemental selenium was produced in these periods. From the TEM images and polyacrylamide gel electropheresis,it is known that the Se0 granule formation is a biologically-induced type,and the cell envelopes are the main biomineralization positions,and particles may go through a process from nucleation to crystallization,under the control of microbes. In fact,the minerals are spherical nanoparticles,occurring as a microcrystal or amorphous form. It is vital to recognize which kinds of proteins and/or polysaccharides act as a template to direct nanoparticle nucleation and growth? This should focus for further studies. This study may shed light on the process of formation of Se(0) nanosphere.


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  • Boskey, A. L., 1998. Biomineralization: Conflicts, Challenges, and Opportunities. Journal of Cellular Biochemistry, 30/31(Suppl. ): 83–91
    Butler, C. S., Debieux, C. M., Dridge, E. J., et al., 2012. Biomineralization of Selenium by the Selenate-Respiring Bacterium Thauera Selenatis. Biochemical Society Transactions, 40: 1239–1243 doi: 10.1042/BST20120087
    Chen, J., Yao, S., 2005. Geomicrobiology and Its Progress. Geological Journal of China Universities, 11: 154–166 (in Chinese with English Abstract)
    Debieux, C. M., Dridge, E. J., Mueller, C. M., et al., 2011. A Bacterial Process for Selenium Nanosphere Assembly. Proceedings of the National Academy of Sciences of the United States of America, 108: 13480–13485 doi: 10.1073/pnas.1105959108
    Dobias, J., Suvorova, E. I., Bernier-Latmani, R., 2011. Role of Proteins in Controlling Selenium Nanoparticle Size. Nanotechnology, 22(19): 195605 doi: 10.1088/0957-4484/22/19/195605
    Dowdle, P. R., Oremland, R. S., 1998. Microbial Oxidation of Elemental Selenium in Soil Slurries and Bacterial Cultures. Environmental Science & Technology, 32: 3749–3755 doi: 10.1021/es970940s
    Ehrlich, H. L., Newman, D. K., 2008. Geomicrobiology. CRC Press: Boca Raton, FL. 656
    Frankel, R. B., Bazylinski, D. A., 2003. Biologically Induced Mineralization by Bacteria. Biomineralization, 54: 95–114
    Kaur, G., M., Iqbal, M., Bakshi, M. S., 2009. Biomineralization of Fine Selenium Crystalline Rods and Amorphous Spheres. Journal of Physical Chemistry C, 113: 13670–13676 doi: 10.1021/jp903685g
    Kessi, J., Hanselmann, K. W., 2004. Similarities between the Abiotic Reduction of Selenite with Glutathione and the Dissimilatory Reaction Mediated by Rhodospirillum Rubrum and Escherichia Coli. Journal of Biological Chemistry, 279: 50662–50669 doi: 10.1074/jbc.M405887200
    Kessi, J., Ramuz, M., Wehrli, E., et al., 1999. Reduction of Selenite and Detoxification of Elemental Selenium by the Phototrophic Bacterium Rhodospirillum Rubrum. Applied and Environmental Microbiology, 65: 4734–4740 doi: 10.1128/AEM.65.11.4734-4740.1999
    Knoll, A. H., 2003. The Geological Consequences of Evolution. Geobiology, 1: 3–14 doi: 10.1046/j.1472-4669.2003.00002.x
    Lenz, M., Kolvenbach, B., Gygax, B., et al., 2011. Shedding Light on Selenium Biomineralization: Proteins Associated with Bionanominerals. Applied and Environmental Microbiology, 77: 4676–4680 doi: 10.1128/AEM.01713-10
    Lenz, M., Lens, P. N. L., 2009. The Essential Toxin: The Changing Perception of Selenium in Environmental Sciences. Science of the Total Environment, 407: 3620–3633 doi: 10.1016/j.scitotenv.2008.07.056
    Lei, L., 2010. Microbial Geochemistry of Selenium in Se-Rich Carbonaceous Mudstone from Yutangba, Enshi of Hubei Province in China: [Dissertation]. Graduate University Chinese Academy of Sciences, Beijing (in Chinese with English Abstract)
    Lloyd, J. R., Pearce, C. I., Coker, V. S., et al., 2008. Biomineralization: Linking the Fossil Record to the Production of High Value Functional Materials. Geobiology, 6: 285–297 doi: 10.1111/j.1472-4669.2008.00162.x
    Lowenstam, H. A., 1981. Minerals Formed by Organisms. Science, 211: 1126–1131 doi: 10.1126/science.7008198
    Lu, A. H., 2007. Mechanisms of Environmental Response to Biomineralization. Geological Journal of China Universities, 13: 613–620 (in Chinese with English Abstract)
    Mann, S., 1993. Biomineralization—The Hard Part of Bioinorganic Chemistry. Journal of the Chemical Society-Dalton Transactions, 1–9. doi: 10.1039/DT9930000001
    Mann, S., 2001. Biomineralization: Principles and Concepts in Bioinorganic Materials Chemistry. Oxford University Press, New York.
    Oremland, R. S., Herbel, M. J., Blum, J. S., et al., 2004. Structural and Spectral Features of Selenium Nanospheres Produced by Se-Respiring Bacteria. Applied and Environmental Microbiology, 70: 52–60 doi: 10.1128/AEM.70.1.52-60.2004
    Pearce, C. I., Pattrick, R. A. D., Law, N., et al., 2009. Investigating Different Mechanisms for Biogenic Selenite Transformations: Geobacter Sulfurreducens, Shewanella Oneidensis and Veillonella Atypica. Environmental Technology, 30: 1313–1326 doi: 10.1080/09593330902984751
    Rehr, J. J., Ankudinov, A. L., 2001. Progress and Challenges in the Theory and Interpretation of X-Ray Spectra. Journal of Synchrotron Radiation, 8: 61–65 doi: 10.1107/S0909049500016423
    Stolz, J. F., Oremland, R. S., 1999. Bacterial Respiration of Arsenic and Selenium. Fems Microbiology Reviews, 23: 615–627 doi: 10.1111/j.1574-6976.1999.tb00416.x
    Weiner, S., Dove, P. M., 2003. An Overview of Biomineralization Processes and the Problem of the Vital Effect. Biomineralization, 54: 1–29
    Yuan, Y. Q., Zhu, J. M., Liu, C., et al., 2014. Three High-Reducing Selenite-Tolerance Bacteria from Se-Laden Carbonaceous Mudstone. Earth Science Frontiers, 21(1): (in Chinese with English Abstract)
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