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Volume 36 Issue 4
Aug 2025
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Article Contents
Xianxin Huang, Yanhong Wang, Helin Wang, Xinyan Shi, Chunlei Huang, Hanqin Yin, Yixian Shao, Ping Li. A Novel Selenite-Reducing Bacterium Bacillus pseudomycoides SA14 Isolated from Se-Enriched Soil and Its Potential Se Biofortification on Brassica chinensis L.. Journal of Earth Science, 2025, 36(4): 1756-1765. doi: 10.1007/s12583-022-1676-3
Citation: Xianxin Huang, Yanhong Wang, Helin Wang, Xinyan Shi, Chunlei Huang, Hanqin Yin, Yixian Shao, Ping Li. A Novel Selenite-Reducing Bacterium Bacillus pseudomycoides SA14 Isolated from Se-Enriched Soil and Its Potential Se Biofortification on Brassica chinensis L.. Journal of Earth Science, 2025, 36(4): 1756-1765. doi: 10.1007/s12583-022-1676-3

A Novel Selenite-Reducing Bacterium Bacillus pseudomycoides SA14 Isolated from Se-Enriched Soil and Its Potential Se Biofortification on Brassica chinensis L.

doi: 10.1007/s12583-022-1676-3
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  • Corresponding author: Li Ping, pli@cug.edu.cn
  • Received Date: 19 Mar 2022
  • Accepted Date: 26 Apr 2022
  • Available Online: 05 Aug 2025
  • Issue Publish Date: 30 Aug 2025
  • Microbial participation in biofortification can improve the availability of selenium (Se) in soil and contribute to the enrichment of Se in crops. In this study, a selenite (Se(Ⅳ)) reducing strain was isolated from Se-rich soil, and its Se transformation and bio-enhancement ability were studied. The strain was identified as Bacillus pseudomycoides and could reduce more than 93.48% of 1.0 mM Se(Ⅳ) in 54 h. The results of scanning electron microscope (SEM) and energy dispersive X-ray spectrometry (EDS) showed that Se(Ⅳ) was reduced to Se(0), and Se nanoparticles (SeNPs) were eventually formed. In pot experiments, B. pseudomycoides SA14 could promote the bioavailable Se in soils and the concentration of Se in Brassica chinensis L.. The concentrations of water-soluble Se, ion exchange Se and carbonate-binding Se in soil were increased by 23.13%, 22.05% and 30.89%, respectively. The Se concentration of Brassica chinensis L. in pot experiments was increased by 145.05%. The relative abundance of Bacillus in soil increased from 0.97% to 2.08% in the pot experiments. As far as we know, this is the first report of Se reduction by B. pseudomycoides. This study might provide a prospective strategy for microbial fortification of Se in crops.

     

  • Conflict of Interest
    The authors declare that they have no conflict of interest.
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