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Tectonic activity shapes geothermal systems, yet its influence on microbial biogeography remains poorly resolved. Here, we investigate how tectonic activity influences geothermal systems and their microbial communities by comparing hot springs situated along convergent (Yunnan-Xizang) and divergent (Baikal Rift) margins. The results show that hydrochemistry and microbial community in these two tectonically different regions exhibit pronounced differences: Yunnan-Xizang hot springs, shaped by deep crustal interactions, show high concentrations of Na+, K+, and Cl- ions and are dominated by thermophilic microbes such as
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