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Volume 34 Issue 1
Feb 2023
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Journal of Earth Science  > 2023  >  34(1): 145-155.
Jiyu Chen, Qiang Li, Qiufang He, Heinz C. Schröder, Zujun Lu, Daoxian Yuan. Influence of CO2/HCO3- on Microbial Communities in Two Karst Caves with High CO2. Journal of Earth Science, 2023, 34(1): 145-155. doi: 10.1007/s12583-020-1368-9
Citation: Jiyu Chen, Qiang Li, Qiufang He, Heinz C. Schröder, Zujun Lu, Daoxian Yuan. Influence of CO2/HCO3- on Microbial Communities in Two Karst Caves with High CO2. Journal of Earth Science, 2023, 34(1): 145-155. doi: 10.1007/s12583-020-1368-9
shu

Influence of CO2/HCO3- on Microbial Communities in Two Karst Caves with High CO2

doi: 10.1007/s12583-020-1368-9
  • 1.

    Key Laboratory of Karst Dynamics, MNR & GZAR, Institute of Karst Geology, Chinese Academy of Geological Sciences, Guilin 541004, China

  • 2.

    International Research Center on Karst under the Auspices of UNESCO, Guilin 541004, China

  • 3.

    College of Life Science, Guangxi Normal University, Guilin 541006, China

  • 4.

    Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guilin 541004, China

  • 5.

    School of Geographical Sciences, Southwest University, Chongqing 400715, China

  • 6.

    Institute for Physiological Chemistry, University Medical Center of Johannes Gutenberg University, Mainz 55128, Germany

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    Abstract

  • There is limited knowledge about microbial communities and their ecological functions in karst caves with high CO2 concentrations. Here, we studied the microbial community compositions and functions in Shuiming Cave ("SMC", CO2 concentration 3 303 ppm) and Xueyu Cave ("XYC", CO2 concentration 8 753 ppm) using Illumina MiSeq high-throughput sequencing in combination with BIOLOG test. The results showed that Proteobacteria, Actinobacteria and Bacteroidetes were dominant phyla in these two caves, and Thaumarchaeota was the most abundant in the rock wall samples of SMC. The microbial diversity in the water samples decreased with increasing HCO3- concentration, and it was higher in XYC than that in SMC. The microbial community structures in the sediment and rock wall samples were quite different between the two caves. High concentrations of CO2 can reduce the microbial diversity on the rock walls in karst caves, probably through changing microbial preference for different types of carbon sources and decreasing the microbial utilization rate of carbon sources. These results expanded our understanding of microbial community and its response to environments in karst caves with high CO2.

     

    • Electronic Supplementary Materials: Supplementary materials (Tables S1–S3, Figs. S1–S6) are available in the online version of this article at https://doi.org/10.1007/s12583-020-1368-9.
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